Fig. 2: DpPorA for single-molecule sensing.
From: Assembly of transmembrane pores from mirror-image peptides
a Chemical structure of cationic am8γCD. Interaction of am8γCD with single DpPorA (10 μM, cis) at −50 mV and (10 μM, trans) at +50 mV. Inset shows the corresponding current amplitude histogram. b Chemical structure of neutral γCD and interaction with single DpPorA (100 μM, cis) at −50 mV. Chemical structure of anionic s8γCD and interaction with single DpPorA (100 μM, cis) at +50 mV. c Interaction of nonaarginine (R9) with single DpPorA (10 μM, cis) at −25 mV and −50 mV. Insets show corresponding dissociation dwell time (τoff) and association dwell time (τon) dwell time histograms of R9 blocking fitted with a monoexponential probability function. Interaction of nonaaspartate (D9) with single DpPorA (10 μM, cis) at +50 mV (left). d Electrical recordings showing the reversible chemical modification of 1 mM MePEG-OPSS-1k with DpPorA at +50 mV and the addition of 10 mM DTT resulted in the pore opening. Electrical recordings showing no chemical modification of 1 mM MePEG-OPSS-5k with DpPorA at +50 mV. The current signals (a, b) were filtered at 2 kHz and sampled at 10 kHz. The current signals (c) were digitally filtered at 7 kHz. The current signals d were digitally filtered at 500 Hz.
