Fig. 4: Dose-dependent therapeutic effect of IgETRAP in combination with B. longum in a food allergy model causing acute diarrhea.
a Experimental schedule for an OVA-induced food allergy model and administrations of IgETRAP and B. longum. B. longum (5 × 109 cfu head−1) was administered every two or three days from the day after 1st oral administration of OVA. i.p., intraperitoneal; i.g., intragastric. b Suppression of allergy-induced diarrhea by IgETRAP alone and IgETRAP in combination with B. longum. The experiments were independently performed twice and a representative graph is shown. c, d free IgE (c) and MCPT-1 (d) levels in serum (n = 10 mice in groups of OVA (Red circle), OVA + IgETRAP 10 μg (Green square), OVA + IgETRAP 10 μg + B. longum (Orange triangle), OVA + IgETRAP 100 μg (Purple triangle), and OVA + IgETRAP 100 μg + B. longum (Blue diamond)). Statistical analyses were performed by Kaplan–Meier survival curve analysis with log-rank (Mantel-Cox) test to OVA group (b) and unpaired two-tailed Student’s t-test (c, d). All data are presented as means values ± SEM. Specific p-values are indicated in the figure. Source data are provided as a Source Data file.
