Fig. 5: Efb is K27-ubiquitinated at K71 by host RNF114. | Nature Communications

Fig. 5: Efb is K27-ubiquitinated at K71 by host RNF114.

From: Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3

Fig. 5

a Immunoblots of IP products of WCL from MH-S infected with ΔEfb + Flag − Efb for indicated times (MOI = 2 5). b, c Immunoblots of WCL and IP products from WCL of HEK 293 T cells transfected with indicated plasmids. d Immunoblots of IP products of WCL from MH-S infected with ΔEfb + Flag − Efb for indicated times (MOI = 25). e Immunoblots of WCL and IP products from HEK293T cells transfected with indicated plasmids. f Immunoblots of WCL and IP products from MH-S or RNF114 knockdown (KD) MH-S infected with ΔEfb + Flag − Efb for indicated times (MOI = 25). g Immunoblots of WCL and IP products from HEK293T cells transfected with indicated plasmids. K71R, replace 71 lysine of Efb with arginine. h Immunoblots of WCL and IP products from MH-S infected with ΔEfb + Flag − Efb or ΔEfb + Flag − Efb K71R for indicated times (MOI = 25). Data are representative of three experiments with at least three independent biological replicates. Source data are provided as a Source Data file.

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