Fig. 1: Gene expression signatures of senescence and inflammation are differentially regulated in the aged mouse brain across sexes and brain regions.

a NanoString nCounter transcriptional profiling was used to assess age-, sex, and brain region-dependent differences in gene expression for inflammation- and senescence-related genes (n = 5 for young female and male groups, n = 6 for old female and male groups, with the same brains studied per hippocampus [HIP], subventricular zone-enriched striatum [SVZ], and cerebellum [CERE] sample). Black numbers indicate the total number of young versus old differentially expressed genes per region and sex. Red numbers indicate the number of genes that were distinctly expressed as a function of age in a single brain region and sex (linear regression model, false discovery rate (FDR) q < 0.05). b 15 genes were significantly different in young versus old comparisons across all brain regions and both sexes (β-value, indicative of the degree of change in outcome variable for every 1-unit of change in predictor variable; q < 0.05). Comparison of genes significantly up- or down-regulated as a function of age revealed transcripts that were commonly altered across more than one region or sex comparison (black) or distinctly altered in a single age, sex, and region comparison (red) in the c hippocampus, d subventricular zone, or e cerebellum. f Comparative Ingenuity pathway analysis (IPA) analysis of total probed inflammation- and senescence-related genes revealed z-scores for canonical pathways predicted to be up- (yellow) or down- (blue) regulated in the old versus young brain per region and sex. Source data are provided as a Source Data file.