Fig. 6: The DNA methylation signature for RB treatment outcome prediction.
From: Characterizing DNA methylation signatures of retinoblastoma using aqueous humor liquid biopsy
A The selected panel of 320 differentially methylated probes between Clusters A and B in Fig. 5D can separate Subtype 1 (blue) and Subtype 2 (orange) RB samples from the GSE58783 cohort by Liu et al.36. By comparison, Clusters A and B are identified by green and red bars, respectively. B Venn diagrams showing the overlap of Cluster A (green) with Subtype 1 tumors (blue) and Cluster B (red) with Subtype 2 (orange) tumors. C, D Boxplots showing candidate gene DNA hypo- (C) and hyper-methylation (D) in Cluster B (red) RBs compared to Cluster A (dark green) tumors. The DNA methylation status of each gene in normal retina is shown in light green. For each box plot, the line inside the box denotes the median value, the box denotes the Q3 (top boundary) and Q1 (bottom boundary), and the whiskers denotes the maximum (top) and minimum (bottom) respectively. The P value was calculated using two-sided Welch’s t-tests. Source data are provided as a Source Data file.
