Fig. 2: Schematic of engineering strategies to optimize β-ODAP production in E. coli. | Nature Communications

Fig. 2: Schematic of engineering strategies to optimize β-ODAP production in E. coli.

From: Biosynthesis of plant hemostatic dencichine in Escherichia coli

Fig. 2: Schematic of engineering strategies to optimize β-ODAP production in E. coli.

a Optimization of L-DAP production (Module I). b Comparison of the efficiency of different combinations in oxalyl-CoA (Module II) and β-ODAP (Module III) biosynthesis by feeding experiments. c Enhancing β-ODAP production by improving LsBAHD solubility. d Enhancing β-ODAP production by deleting glyoxylate degradation pathways (aceB, glcB, ycdW, ghrB, gcl). e De novo biosynthesis of β-ODAP by three different artificial pathways. f Enhancing β-ODAP production by synergy of the glyoxylate oxidation pathway and the oxaloacetate cleavage pathway. g Enhancing β-ODAP production by deleting serB. h Enhancing β-ODAP production by overexpressing aceA. The blocked pathways are labeled with red crosses. Solid arrows indicate single-step reactions, and dashed arrows indicate multi-step reactions. The area of the circles for L-DAP and β-ODAP is proportional to their titer.

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