Fig. 6: TM9SF4 silencing suppresses tumor initiation/growth and metastasis by A2780 cells in athymic nude mice.
From: TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis
a, b TM9SF4 knockdown inhibited tumor initiation/growth. Control or TM9SF4 knockdown A2780 cells were injected subcutaneously into the hind leg of nude mice (n = 8 per group), followed by measurement of tumor sizes every 4 days. Shown are the dissected tumors. c–g TM9SF4 knockdown inhibited tumor metastasis to peritoneal cavity and lung. Control or TM9SF4 knockdown A2780 cells were injected intraperitoneally, followed by measurement of peritoneal tumor nodules in number and weight on day 30 (c, d) (n = 11 per group). Control or TM9SF4 knockdown A2780 cells were injected via ail vein, followed by measurement of lung tumor nodule number on day 32 (e–g) (n = 6 per group). Shown are representative images of peritoneal cavity with tumor nodules (d) and dissected lung tissue with tumor nodules (e), and summary data (d, f). g Representative images of H&E staining (upper row) and TM9SF4 immunoreactivity (lower row) of lung tissue sections from e. Scale bar = 200 μm (upper row) and 100 μm (lower row). T stands for metastasis tumor nodules. Data are presented as mean ± SEM and two-tailed unpaired Student’s t-test were used for statistical analysis (b, d, f). Source data are provided as a Source Data file.
