Fig. 7: Effect of CTSE and TKTL1 overexpression on anticancer activity of DOS and XELOX in gastric cancer cell lines MKN45 and MGC803. | Nature Communications

Fig. 7: Effect of CTSE and TKTL1 overexpression on anticancer activity of DOS and XELOX in gastric cancer cell lines MKN45 and MGC803.

From: Proteomic characterization of gastric cancer response to chemotherapy and targeted therapy reveals potential therapeutic strategies

Fig. 7

a. b Effect of CTSE overexpression on proliferation in gastric cancer (GC) cell lines (MKN45 and MGC803, n = 3 independent experiments, two-sided Student’s t test, mean ± SD). c–e Dose-response curves of MKN45 and MGC803 cell lines overexpressing CTSE after 72-h treatments with DOS, XELOX, and DOC. IC50, half-maximal inhibitory concentration. *P-values are calculated using two-sided Student’s t test. Barplots showing the comparison of IC50 values in each group. f, g KEGG pathway analysis showing the differential function in the CTSE group with or without DOC treatment (two-sided Fisher’s exact test). h Immunoblot analysis of soluble alpha-tubulin and soluble beta-tubulin in MKN45 cells after DOC treatment, and the normalization of qualified western blots (n = 3 independent experiments, two-sided Student’s t test, mean ± SD). i, j Effect of TKTL1 overexpression on MKN45 and MGC803 cell proliferation (n = 3 independent experiments, two-sided Student’s t test, mean ± SD). k–m Dose-response curves of MKN45 and MGC803 cell lines overexpressing TKTL1 after 72-h treatments with DOS, XELOX, and DOC (n = 3 independent experiments, two-sided Student’s t test, mean ± SD). IC50, half-maximal inhibitory concentration. Barplots showing the comparison of IC50 values in each group. n, o Chromosome segregation defects (n) and percentage of aneuploid cells (o) in DOC-treated MKN45 cells with or without TKTL1 overexpression. The left panel shows the representative results. The scale bar indicates 10 μm. The right panel shows the statistical results from n = 3 independent experiments in the MKN45 cells (two-sided Student’s t test, mean ± SD). p Schematic illustration indicating how CTSE inducts microtubule stabilization, and how TKTL1 induces abnormal chromosome segregation. Bars represent the mean of n = 3 independent experiments with error bars indicating SD (for c–e, h, k–m, n–o). Source data are provided as a Source Data file.

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