Fig. 5: BSA-Man@Mn2+-Ft@Lap nanoassembly promotes DC maturation and cross-priming.
From: A protein-based cGAS-STING nanoagonist enhances T cell-mediated anti-tumor immune responses
a–c Flow cytometric analysis of the expression levels of mature DC markers CD80/CD86 and effector T cell markers CD4/CD8 and CD8a/ IFN-γ in splenic immune cells co-incubated with 4T1 cells treated with: (I) control, (II) BSA-Man-Ft, (III) BSA-Man-Ft@Lap, (IV) BSA-Man@Mn2+-Ft, or (V) BSA-Man@Mn2+-Ft@Lap for 24 h; flow cytometry experiments in panels a–c were repeated three times independently with similar results. d–g Secretion levels of immunostimulatory cytokines including IFN-γ, TNF-α, IL-6, and IL-10 in the supernatant from the above co-culture system with different treatments, including (I) control, (II) BSA-Man-Ft, (III) BSA-Man-Ft@Lap, (IV) BSA-Man@Mn2+-Ft and (V) BSA-Man@Mn2+-Ft@Lap. Data are presented as mean values ± SEM (n = 5 biologically independent samples for panels d–g). Statistical analysis in panels d–g was carried out via one-way ANOVA method. * Indicates significance at p < 0.05, *** indicates significance at p < 0.001, ****indicates significance at p < 0.0001. Source data are provided as a Source data file.
