Table 4 Whole-cell voltage clamping of control and Tmem65 KD cardiomyocytes

From: Tmem65 is critical for the structure and function of the intercalated discs in mouse hearts

Unite (pA/pF)

Scrambled shRNA (n = 16)

Tmem65 shRNA (n = 25)

Peak INa+

−15.87 ± 0.80

−16.83 ± 1.78

 INa+ activation (mV)

−46.37 ± 0.59

−42.03 ± 0.43*

 INa+ activation slope

5.06 ± 0.52

5.63 ± 0.38

 INa+ inactivation (mV)

−75.51 ± 0.21

−74.70 ± 0.19

 INa+ inactivation slope

−5.20 ± 0.19

−5.67 ± 0.16

Peak ICa2+ (pA/pF)

−7.73 ± 0.22

−5.10 ± 0.35

 ICa2+ activation (mV)

−13.32 ± 0.32

−11.33 ± 0.24*

 ICa2+ activation slope

5.83 ± 0.28

6.86 ± 0.22*

 ICa2+ inactivation (mV)

−27.75 ± 0.39

−23.88 ± 0.18*

 ICa2+ inactivation slope

−6.67 ± 0.25

−6.54 ± 0.16

Unit (pA/pF)

Scrambled shRNA (n = 10)

Tmem65 shRNA (n = 10)

Total IK+, outward

30.44 ± 1.86

41.50 ± 2.45*

 Total Ito

9.81 ± 1.85

25.25 ± 2.39*

 Total IK+, slow1

8.19 ± 1.19

5.13 ± 0.77*

 Total IK+, slow2

8.15 ± 0.57

7.51 ± 0.73

 Total Iss

4.02 ± 0.35

4.25 ± 0.46

IK+, outward (4-AP-sensitive)

16.6 ± 0.96

18.45 ± 1.12

 Ito (4-AP-sensitive)

7.21 ± 1.28

15.96 ± 1.19*

 IK+, slow1 (4-AP-sensitive)

6.24 ± 0.96

1.36 ± 0.47**

 IK+, slow2 (4-AP-sensitive)

4.40 ± 0.46

3.18 ± 0.50

 Iss (4-AP-sensitive)

0.07 ± 0.05

0.375 ± 0.11*

IK+, outward (4-AP-insensitive)

15.00 ± 2.01

22.60 ± 1.90*

 Ito (4-AP-insensitive)

3.55 ± 0.84

8.95 ± 1.31*

 IK+, slow1 (4-AP-insensitive)

3.35 ± 0.57

4.59 ± 0.56**

 IK+, slow2 (4-AP-insensitive)

4.40 ± 0.60

5.62 ± 0.52

 Iss (4-AP-insensitive)

4.42 ± 0.83

3.84 ± 0.37

  1. n = 16 control and 25 Tmem65 KD cardiomyocytes for in INa+ and ICa measurements. n = 10 cardiomyocytes per group for Ik+ measurement. All cells were isolated from male mouse hearts.
  2. mV millivolt, 4-AP 4-aminopyridine.
  3. *p  < 0.05, **p < 0.01.
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