Fig. 5: In vivo fluorescence imaging with subcutaneous injected Au-PC, Au-GSH and ICG. | Nature Communications

Fig. 5: In vivo fluorescence imaging with subcutaneous injected Au-PC, Au-GSH and ICG.

From: Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models

Fig. 5: In vivo fluorescence imaging with subcutaneous injected Au-PC, Au-GSH and ICG.

Wide-field NIR-II fluorescence images (excited by an 808 nm laser at a power density of 70 mW/cm2, exposure times 100 ms and 4 ms for Au and ICG, respectively, 1100 nm long pass filter) of bilateral subcutaneously (s.c.) injected a Au-PC conjugate (4x, ~ 1.2 mg), b Au-GSH cluster (4x, ~ 1.2 mg) and c ICG (50 µL, 50 mM) probes at different time points (six to seven weeks old female Balb/c, n=3). Normalized fluorescence intensities (left Y axes) of Right (R) inguinal lymph nodes (iLNs) up to six hours postinjection and region of interest signal (ROI, right Y axes) around the injection site up to 24 h post-injection of Au-PC (d), Au-GSH (e) and ICG (f) fluorescent probes. Error bars represent standard deviation (SD) of three repeated experiments. Data are presented as mean values ± SD. Source data are provided in Source Data file.

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