Fig. 6: Lymph node imaging in various NIR subwindows. | Nature Communications

Fig. 6: Lymph node imaging in various NIR subwindows.

From: Phosphorylcholine-conjugated gold-molecular clusters improve signal for Lymph Node NIR-II fluorescence imaging in preclinical cancer models

Fig. 6

Wide-field fluorescence images (excited by an 808 nm laser at a power density of 70 mW/cm2) of intra-tumoral (i.t.) injected a 1x Au-PC conjugate and b ICG probes into a mouse bearing 4T1 tumor (six weeks old female Balb/c, n=3). The images present the right lateral view taken 30 min p.i and 3 h p.i for Au-PC and ICG respectively at different NIR-I and NIR-II windows. The exposure times for detecting >900 nm, >1100 nm, > 1200 nm and > 1300 nm emission were 25 ms, 20 ms, 90 ms and 400 ms for Au-PC and 0.4 ms, 3 ms, 15 ms, 100 ms for ICG respectively. c Fluorescence intensity cross-sectional profile of iLN after Au-PC administration. Locations of LN signal and background (B) on the line profiles are marked with arrows. (d) The comparison of lymph node signal-to-background (LN/B) ratios of Right (R) inguinal lymph node (iLNs) at different NIR-I and NIR-II sub-windows. Error bars represent standard deviation of three repeated experiments. Source data are provided in Source Data file.

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