Fig. 4: Gene expression in peripheral blood mononucleated cells (PBMCs) during short-term fasting.
From: Fatty acids homeostasis during fasting predicts protection from chemotherapy toxicity
a, b Total RNA from PBMCs isolated from human volunteers (a) or mice (b) at the indicated time points (B basal, F fasting, R refeeding) was analyzed for the expression of the indicated lipid metabolism genes. c, d Heatmaps depicting the Pearson correlation r coefficients in humans (c) or mice (d) between the changes with fasting of erythrocyte membrane fatty acids and/or circulating miRNAs, and the changes in mRNA expression of the indicated genes identified in the PBMC RNAseq experiment. e, f Heatmaps depicting each individual, represented by a number next to each panel, and its position with respect to the median value for each of the indicated parameters: red indicates “above the median”, and blue indicates “below the median”. Both individuals and parameters were ordered according to their similarity. This unsupervised clustering defined two groups of individuals named “A” and “B” in the graphs. Line-connected dots represent the paired data for each individual (a, b). Statistical analysis was performed using the one-way ANOVA test with Tukey correction for multiple comparisons (a); the paired two-tailed Student t test (b); or Pearson correlations (c, d). The exact p value is provided for each significant comparison. Source data are provided as a Source Data file.
