Fig. 4: LGE-like origin of inhibitory neurons in 1-month-old SNR-derived organoids. | Nature Communications

Fig. 4: LGE-like origin of inhibitory neurons in 1-month-old SNR-derived organoids.

From: Modeling human telencephalic development and autism-associated SHANK3 deficiency using organoids generated from single neural rosettes

Fig. 4

a Heatmap visualization of the Jaccard similarity index between unique DEGs in the human fetal neocortex (CTX), lateral ganglionic eminence (LGE) and medial ganglionic eminence (MGE) at 7, 9, and 11 post-conception weeks (PCW)8 and those in different clusters of 1-month-old SNR-derived organoids (Supplementary Data 4). b UMAP visualization of expression of LGE-specific marker genes, GSX2, SALL3, RP11-849l19.1, MEIS2, PBX3, and FOXP2 in 1-month-old organoids. c Organoid sections immunostained for PAX6 and GSX2. d Quantification of percentages of PAX6-, GSX2- and PAX6/GSX2-expressing cells in the lumen-surrounding VZ (n = 7 organoids/1–2 sections per organoid in two batches from 2242-5 and EP2-15 lines). e Angular distributions of PAX6-, GSX2- and PAX6/GSX2-expressing cells in the VZ (n = 3 organoids/1 sections per organoid from 2242-5 line). f, g Images of fetal human brain tissue sections at 9 (f) and 11 (g) PCW stained using FISH probes against PAX6 and GSX2 (f) or immunostained with antibodies against PAX6 and GSX2 (g). h Organoid sections immunostained for CTIP2 and GABA. i Percentage of inhibitory neurons (GABA- or GAD67 expressing cells) that co-expressed CTIP2 (n = 11 organoids/1–5 sections per organoid in 3 batches from H9 and 2242–5 lines). Data are means ± s.e.m. Scale bars = 100 and 50 (zoom-in) (c, h), 500 (f), and 200 (f, g) μm. Source data are provided as a Source Data file.

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