Fig. 3: Spatially resolved functional gene signatures of lung cancer metastasis.

a Heatmap of functional gene signatures (Fges) to show changes in the cellular or extracellular components in the different regions of the primary and metastasized tumors. ROIs from the same region are grouped together and distinguished by different color codes at the top of graph. The boxes with broken lines highlight gene clusters with significant differences in expression between the TIME-L and the TIME-B or TBME. The boxes with dotted lines denote gene clusters with significant differences between the TBME and BC groups. b Heatmap of pEMT signature genes across the ROIs. The box with broken lines highlights a gene cluster with significant difference between the tumor cores (L, LB) and the tumor microenvironment (TIME-L/B and TBME). The box with dotted lines denotes a gene cluster with similar expression patterns between the TBME and BC. Heatmaps colored from blue to red according to Z-score scale −2 to 2. Source data are provided as a Source Data file.