Fig. 3: Non-canonical processing of mitochondrial gene junctions results in the formation of 3′ phosphates that are hydrolysed by DmANGEL or MmANGEL2.
From: ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing
a Mitochondrial polyadenylation tail (MPAT) length assay in MmAngel2KO mouse heart samples, performed with or without CIP or PNK pre-treatment, as indicated. b Mitochondrial polyadenylation tail (MPAT) length assay in DmAngelKO fly samples, performed with or without CIP or PNK pre-treatment, as indicated. CIP calf intestinal phosphatase, hydrolyses phosphomonoester bonds. PNK T4 polynucleotide kinase, hydrolyses phosphomonoester bonds or 2′,3′ cyclic phosphodiester from RNA ends. Gene junctions and their sequences are indicated. Non-coding sequences are lower case. Sequences not annotated are shown in lower case grey. tRNAs are shown in orange with their single letter code. Poly(A) tails (-An) and 3′ phosphates (-℗) are shown. A representative experiment is shown of at least three independent experiments performed with biologically independent samples.
