Fig. 4: ANGEL2 and DmANGEL are terminal phosphatases in vivo and in vitro. | Nature Communications

Fig. 4: ANGEL2 and DmANGEL are terminal phosphatases in vivo and in vitro.

From: ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing

Fig. 4: ANGEL2 and DmANGEL are terminal phosphatases in vivo and in vitro.The alternative text for this image may have been generated using AI.

MPAT length assay using RtcB ligase in a MmAngel2KO mouse heart or b DmAngelKO fly samples. c In vitro phosphatase activity of recombinant Drosophila (DmAngel), mouse (MmANGEL), or human (HsANGEL) ANGEL or ANGEL2 protein, in the presence of RNA oligos (20 nts) containing 3′-, 2′-, or 2′,3′-cPs as indicated. PNK treatment was used as positive control. Representative experiments are shown of at least two independent experiments performed with biologically independent samples.

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