Fig. 6: Members of the FASTK family are required for non-canonical processing of mitochondrial transcripts.
From: ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing
a Volcano plot of proteomics data from immunoprecipitation (IP) experiments, using 4 dael larvae from DmANGEL-FLAG versus DmANGEL overexpressing larvae (n = 4). Proteins in the category “mitochondria” are highlighted in blue. Limma moderated t-test, p values are given as false discovery rate after adjustment for multiple testing (n = 4 biologically independent samples). b Heatmap of the enrichment of DmANGEL and CG13850 in CG13850-FLAG IP experiments (n = 4 biologically independent samples). c Neighbour-joining protein MUSCLE alignment of 30 FASTK family members (see Supplementary Table 1) from five species (as indicated) shown in an unrouted tree layout. CG13850 and CG31643 are the two Drosophila FASTK family orthologs. d Northern blot analysis of selected mitochondrial transcripts in 4-dael control (daGAL4 and RNAi) larvae and larvae with silenced CG13850 (CG13850 RNAi). RP49 was used as loading control. Representative experiment is shown with n = 2 biologically independent samples, of two independent experiments performed. e BN-PAGE and in-gel activity staining of NADH dehydrogenase (Complex I), cytochrome c oxidase (Complex IV), and ATP synthase (Complex V) with a Coomassie stain as a loading control. Dissociation of the ATPase F1 subunit is indicated by *. Representative experiment is shown with n = 2 biologically independent samples.
