Fig. 2: A subset of super-enhancers is specific to primary CRC specimens and not recapitulated in CRC cell lines. | Nature Communications

Fig. 2: A subset of super-enhancers is specific to primary CRC specimens and not recapitulated in CRC cell lines.

From: A local tumor microenvironment acquired super-enhancer induces an oncogenic driver in colorectal carcinoma

Fig. 2: A subset of super-enhancers is specific to primary CRC specimens and not recapitulated in CRC cell lines.

a PCA of H3K27ac signal at 2026 SEs in CRC (n = 15 independent tissue samples) and CRC cell lines (n = 13 independent cell lines). Please see Methods for accessions. b Heatmap of H3K27ac signal (Z-score) between CRC tumors and CRC cell lines with unsupervised hierarchical clustering and Pearson’s correlation testing. 221 SEs (out of 2026) down-regulated in CRC cell lines compared to primary tumors (mean H3K27ac signal log2 fold change <−1, P < 0.01). c GSEA validation of a tumor-enriched SE gene signature imposed on differentially expressed genes between primary CRC and CRC cell lines. See Supplementary Table 3 for gene list. d Venn diagram showing overlap between 221 SEs down-regulated in CRC cell lines from b with the top 100 gained SEs in primary CRC over patient-matched normal. NES normalized enrichment score, Q false discovery rate.

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