Fig. 6: PDZK1IP1 is a context-dependent regulator of CRC tumor growth.

a, b Growth curves, sgPDZK1IP1 and sgNeg subcutaneous xenograft tumors in nude mice. HT29 n = 10 mice per group, DLD1 n = 10 mice per group. 1 million cells injected per mouse for both HT29 and DLD1. Data presented as mean \(\pm\) s.e.m. Significance was determined using two-sided Student’s t-test. c, d Growth curves, PDZK1IP1-V5 and EV xenograft tumors. HT29 n = 10 mice per group, DLD1 n = 5 mice for PDZK1IP1-V5, n = 8 mice for EV. 3-5 million cells injected for both HT29 and DLD1. Data presented as mean \(\pm\) s.e.m. Significance was determined using two-sided Student’s t test. e Meta-comparison of 2D Incucyte growth curves in vitro with xenograft tumor growth in vivo. KO – sgPDZK1IP1.329, WT – sgNeg, EV – empty vector, OE – PDZK1IP1-V5. ΔGrowth presented as (KO-WT) as percentage of WT, or (OE-EV) as a percentage of EV. Data presented as mean \(\pm\) s.e.m. Significance was determined using two-sided Student’s t-test. HT29 KO and WT, as well as DLD1 KO and WT in vivo (n = 10 mice per group). HT29 KO and WT, as well as DLD1 KO and WT in vitro (n = 6 biological replicates). f Growth curves of HT29 cells with CRISPR dCas9-KRAB interference of the E2 region of the PDZK1IP1 SE (sgE2), and sgNeg subcutaneous xenograft tumors (n = 8 mice per group.) 1 million cells injected per mouse. Data presented as mean \(\pm\) s.e.m. Significance was determined using two-sided Student’s t test. Source data are provided as a Source Data file.