Fig. 3: TRB1 protein is essential for mitochondrial movement and ER association. | Nature Communications

Fig. 3: TRB1 protein is essential for mitochondrial movement and ER association.

From: TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy

Fig. 3

a, b Arabidopsis expressing Mito-mCherry and VAP27-RFP was immuno-labelled with a TRB1 antibody; the endogenous protein localized at the outer mitochondrial membrane, and in contact with VAP27-labelled ER membrane, respectively. c An example of stationary mitochondria (arrow) that stay immobile during the six second time course. d, e The percentage of stationary mitochondria (immobile for at least six seconds) increased significantly in the presence of GFP-TRB1 or VAP27-1. TEM study of wild type Arabidopsis (f) and stable Arabidopsis transformed with GFP-TRB1 (g), the distance between ER and mitochondria is significantly reduced compared to wild type plants (h). a, b Immunolabelling experiments were performed two times with similar results. c–e Microscopic imaging were repeated at least three times with similar results. For mitochondria movement analysis, n = 15 cells; For TEM analysis, at least 20 mitochondria from 15 cells in total examined over three biologically independent samples, error bars are SEM, ** 0.001 < P < 0.01, *** P < 0.001 in two-tailed Student’s t tests and with Welch’s correction in (h) (scale bar = 10 μm for light microscopy; scale bar = 500 nm for TEM).

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