Fig. 1: Glucose induces H3K4me3 via the Ras-cAMP-PKA pathway.

a Effect of glucose on histone modifications. Cells were grown in YPD medium until OD₆₀₀ of 0.7–1.0 followed by treatment with YP medium supplemented with different concentrations of glucose for 0.5 hr. Cells were harvested and the extracted histones were analyzed by Western blots with indicated antibodies. b Effect of 2-Deoxy-D-glucose (2-DG) on H3K4 methylation. c Diagram showing nutrient sensors in budding yeast. d Effect of nutrient sensors on H3K4 methylation. e Western blot analysis of the effect of Tpk1 and Tpk2 on H3K4 methylation. f Tpk2 is required for glucose to induce H3K4me3. g Diagram showing the connection between glycolysis and the Ras-cAMP-PKA pathway. h The global H3K4me3 was increased in pde1∆ and pde2∆ mutants. i Western blot analysis of H3K4 methylation in WT, ras2∆, and gpa2∆ mutants. For d and e, data represent the mean ± SEM of three biological independent experiments. For h, data represent the mean ± SEM of five biological independent experiments. For i, data represent the mean ± SEM of four biological independent experiments. Two-sided t-tests were used for statistical analysis. Source data are provided as a Source data file.