Fig. 7: Tpk2-catalyzed Jhd2 phosphorylation maintains normal chronological life span and promotes autophagy.

a Venn diagram showing genes co-regulated by Jhd2-S321A, S340A and tpk2Δ mutants. b Left panel: GSEA analysis of longevity regulating genes in Jhd2-S321A, S340A and tpk2Δ mutants. Right panel: Heatmap showing the transcription of longevity regulating genes in Jhd2-S321A, S340A and tpk2Δ mutants. c Chronological life span analysis of WT, Jhd2-S321A, S340A and tpk2Δ mutants. d Venn diagram showing genes co-regulated by Jhd2-S321A, S340A and rpd3Δ mutants. e GSEA analysis of autophagy genes regulated by tpk2Δ, Jhd2-S321A, S340A, jhd2Δ and rpd3Δ mutants. f Heatmap showing the binding of Jhd2 and Rpd3 at autophagy genes in WT and Jhd2-S321A, S340A mutant. Heatmap for the transcription of autophagy genes in jhd2Δ, Jhd2-S321A, S340A, tpk2Δ and rpd3Δ mutants was also provided. g ChIP-qPCR analysis of Jhd2 (top panel) and Rpd3 (bottom panel) occupancy at representative autophagy genes in WT, Jhd2-S321A, S340A and tpk2Δ mutants. PYK1 was used as a negative control. h RT-qPCR analysis of representative autophagy genes in WT, Jhd2-S321A, S340A, tpk2Δ, rpd3Δ, rpd3Δ Jhd2-S321A, S340A, and rpd3Δ tpk2Δ mutants. i, j Analysis of autophagy activity in WT, Jhd2-S321A, S340A, tpk2Δ, rpd3Δ, rpd3Δ Jhd2-S321A, S340A, and rpd3Δ tpk2Δ mutants. k Proposed model for regulation of autophagy by Tpk2-catalyzed Jhd2 phosphorylation. b, e, The P value was calculated using a permutation test with 1,000 permutations. c, g–j, Data represent the mean ± SEM of three biological independent experiments. Two-sided t-tests were used for statistical analysis. Source data are provided as a Source data file.