Fig. 6: XPF promotes HR and break-induced telomere synthesis.

a Representative images of TRF2 and BRCA1 immunostaining in U2OS cells transfected with control, XPF, FANCM, or XPF/FANCM siRNAs for 3 days. b Quantification of colocalization events of BRCA1 and TRF2 in (a). c Quantification of colocalization events of RAD51 and TRF2 in U2OS cells transfected with control, XPF, FANCM, or XPF/FANCM siRNAs for 3 days. d Representative images of EdU-DNA-FISH to detect telomere synthesis in siRNA transfected U2OS cells after the treatment of RO-3306. Arrows indicate the colocalization events. e Quantification of EdU and telomere colocalization in non-S phase cells in (d). f Quantification of POLD3 and TRF2 colocalization events in U2OS cells transfected with siRNAs after the treatment of RO-3306. g Quantification of EdU and telomere colocalization events in stable cell lines expressing RCas9-sgRNAs. TERRA knockdown cells (sgTER_1C6, sgTER_1C21) or control cells (sgSense, sgλ2). h Quantification of EdU and telomere colocalization events in U2OS cells with transient TERRA knockdown by viral transduction with RCas9-sgRNA plasmids. i Quantification of EdU and telomere colocalization events in WI38-VA cells transfected with control, XPF, FANCM, or XPF/FANCM siRNAs. a–i Data of three independent experiments. n, cell number. Bars, mean ± SEM. Mean values of each group shown on the top of figures. P values by two-sided Mann-Whitney test.