Fig. 4: Analysis of SC/axis lengths in female and male meiocytes.
From: Joint control of meiotic crossover patterning by the synaptonemal complex and HEI10 dosage
A–D REC8 immunolocalization in female and male meiocytes of wild type and zyp1 HEI10oe homo (Col). Imaging was done with 3D-STED and the projection is shown. Scale bar = 1 µm. E–H REC8 signal was traced in 3D. Each bivalent pair is color-coded. I–L Individual trace of the longest chromosome (presumably chromosome 1), with start-to-end color code. M Measurement of the total SC length. Each dot is the SC length of an individual cell. Circles and triangles are females and males, respectively. The bars indicate the mean. One-way ANOVA followed by Sidak correction showed that SCs were systematically longer in males than in females (p < 0.000001). The same test did not detect any differences between any of the pairs of males of different genotypes (p > 0.7). For females, none of the pairwise comparisons were significantly different (p > 0.13) except in Col/Ler HEI10oe that was lower than Col/Ler zyp1 (p = 0.006) and Col zyp1 (p = 0.008). Note that variations in slide preparation and exact meiotic stage may affect this result. The number n of analyzed cells in indicated. N Correlation analysis between the mean number of COs per chromosome and SC length (µm) in Col/Ler background. SCs were attributed to specific chromosomes based on their length (e.g., the longest was presumably chromosome 1). Pearson’s correlation coefficients are shown in parentheses. The number of samples in shown in Fig. 4M for SC length and in Fig. 1D for crossovers per chromatid. The relationship between the mean number of MLH1 foci per cell and total SC length per cell in O Col background and P Col/Ler background. The number of samples in shown in Fig. 4M for SC length and in Fig. 1B for MLH1 foci per cell. Q The relationship between the mean number of COs and SC length in Col/Ler background. The number of samples in shown in Fig. 4M for SC length and in Fig. 1D for crossovers per chromatid set. The 90% confidence intervals are indicated as error bars. Source data are provided as a Source Data file.
