Fig. 1: Discovery of M1 selective probe by image-based screening.
From: Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
A Metabolic pathways of M1 and M2 macrophages. Whereas M1 macrophages upregulate glucose transporters (GLUTs) to uptake carbohydrates and operate aerobic glycolysis, M2 macrophages highly expressed CD36 to make an energy from OXPHOS and β-oxidation. B 80-membered Luminescent-carbohydrate (LC) library was composed of 4 backbones of carbohydrates conjugated with 20 different physical properties of fluorophores. C Workflow of image-based screening to develop an M1 selective probe. RAW264.7 cells were used as the resting state of macrophages and differentiated into M1 and M2 macrophages. M0, M1, and M2 cells were seeded into 96-well plates, and screened by high-throughput screening microscope. D Chemical structure of CDr17. E, F Selectivity of CDr17 to M1 over M0 or M2 macrophages from RAW264.7 and relative fluorescent intensity (FI) of CDr17. All the images were acquired at ×40 magnification. Data are presented as mean values ± SD (n = 3). All error bars represented standard deviation from three independent measurements. Scale bar, 50 µm. Source data are provided as a Source data file.
