Fig. 3: Target validation of CDr17.
From: Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target
A The scheme of GLUT1 inhibition study with GLUT1 specific inhibitor, STF-31. B The effects of STF-31 on the uptake of CDr17 by images using M1 macrophages from RAW264.7. STF-1 was added to M1 macrophages, and CDr17 (1 μM) was treated for 30 min. CDr17 fluorescent intensity (FI) was plotted into the graph. All the images were acquired at 40x magnification. Data are presented as mean values ± SD. All error bars represented standard deviation from three independent measurements. C Schematic view of GLUT1-CRISPR-knockout (KO) in M1 macrophages. D GLUT1 gene level from control and GLUT1 KO M1 cells. Data pooled from three individual experiments. Data are analyzed with three samples over three independent experiments (n = 3). E The fluorescence intensity of control and GLUT1 KO M1 macrophages. CDr17 (1 μM) was added for 30 min. All the images were acquired at ×40 magnification. Data are presented as mean values ± SD (n = 3). All the error bars represented standard deviation from three independent measurements. All the scale bars represented 50 µm. Source data are provided as a Source data file.
