Fig. 8: Mechanisms of mitochondrial H₂O₂ generation.

a Immunoblots in AT2 mitochondria (left) and densitometric quantification of immunoblots (right) show MCU expression 24 h after indicated treatments. LPS was instilled at a nonlethal dose (1 mg/kg). Cx43, connexin 43; Cx43^F/F, mice floxed for Cx43; AT2^Cx43−/−, mice lacking Cx43 in AT2. Bars: mean ± SEM. n = 3 lungs each bar. Groups were compared using one-way ANOVA with Bonferroni correction. b Determinations of mitochondrial Ca²⁺ responses to hyperinflation 24 h after indicated intranasal instillations. LPS was instilled at a nonlethal dose. Bars: mean ± SEM. n = 4 lungs each bar. Groups were compared using one-way ANOVA with Bonferroni correction. c Immunoblot (left), and densitometric determinations (right) from AT2 mitochondria. AT2 were isolated 24 h after instillations of either PBS or nonlethal LPS. ECSIT, evolutionarily conserved signaling intermediate in Toll pathway; ECSIT^+/−, heterozygous knockout mice for ECSIT. Bars: mean ± SEM. n = 3 lungs each bar. Groups were compared using one-way ANOVA with Bonferroni correction.