Fig. 1: MHC II aAPCs stimulate functional antigen-specific murine CD4+ T cells. | Nature Communications

Fig. 1: MHC II aAPCs stimulate functional antigen-specific murine CD4+ T cells.

From: Nanoparticle-based modulation of CD4+ T cell effector and helper functions enhances adoptive immunotherapy

Fig. 1: MHC II aAPCs stimulate functional antigen-specific murine CD4+ T cells.The alternative text for this image may have been generated using AI.

a Design of MHC II aAPCs with MHC class II molecules (MHC II) as Signal 1 (S1) and anti-CD28 antibodies (αCD28) as Signal 2 (S2). S2 is either attached to aAPCs (S1/2) or delivered solubly (S1 + S2). Created with BioRender.com. b Fluorescent quantification of I-AbOVA and αCD28 conjugated to S1/2 and S1 aAPCs. c OT-II CD4+ T cell fold proliferation after 7 days of stimulation with I-AbOVA S1/2 aAPCs compared to polyclonal αCD3/αCD28 or I-AbCLIP aAPCs. d Day 7 OT-II fold proliferation following treatment with S1 aAPCs and titration of S2, compared to S1/2 or αCD3/αCD28 aAPCs. e Day 7 T-bet staining, f CD4+ lineage transcription factor staining, and g cytokine production of OT-II cells stimulated with S1/2 aAPCs in media containing: no cytokines, IL-2, T cell growth factor (TF) cytokine cocktail, or a Th1 mix (IL-2, IL-12p70, IFN-γ). h Day 7 cytokine production of OT-II cells stimulated with S1/2, S1 + S2, or αCD3/αCD28 aAPCs versus peptide-pulsed OT-II splenocytes or bone marrow-derived dendritic cells (BMDCs). Data in (b–d, f–h) represent the mean ± standard error of the mean (s.e.m.) from three or more independent experiments. b n = 8, c, d n = 4 mice, f n = 3 (no cytokines) or 4 mice (naïve OT-II, IL-2, TF, Th1 mix), g n = 4 mice, h n = 4 (S1/2), 5 (BMDCs), or 6 mice (Naïve, Spleen APCs, αCD3/αCD28, S1 + S2), analyzed using (b) n unpaired Student’s t-test, two-tailed, c, d A one-way ANOVA compared to “no stim.” with Dunnet’s multiple-comparisons test, or f–h a two-way ANOVA with Tukey’s multiple-comparisons test.

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