Fig. 2: MHC II aAPCs expand rare murine CD4+ T cell subsets. | Nature Communications

Fig. 2: MHC II aAPCs expand rare murine CD4+ T cell subsets.

From: Nanoparticle-based modulation of CD4+ T cell effector and helper functions enhances adoptive immunotherapy

Fig. 2: MHC II aAPCs expand rare murine CD4+ T cell subsets.The alternative text for this image may have been generated using AI.

a Schematic of magnetic enrichment of rare CD4+ T cells with aAPCs. Created with BioRender.com. b Representative flow plots (left) and fold enrichment (right) of OT-II cells diluted into a B6 background at a ratio of 1:1000 after magnetic enrichment with S1/2 or S1 aAPCs. c Representative flow plots and d percent of OT-II (cognate) and B6 (non-cognate) CD4+ T cells bound to particles after 2 h of incubation at 37 °C with S1/2 versus S1 aAPCs across a range of doses. e Representative flow plots and f fold expansion of OT-II and SMART-A1 cells diluted 1:1000 into a B6 background, as measured 7 days after S1 + S2 enrichment and expansion. g pMHC Tetramer staining and h quantified a number of I-AbOVA CD4+ T cells 7 days after S1/2 or S1 + S2 enrichment and expansion. Data in b, d, f, h represent mean ± standard error of the mean (s.e.m.) from three or more independent experiments. b n = 4 mice, d n = 3 (S1/2) or 4 (S1) mice, (f.1) n = 6 mice, (f.2) n = 3 mice, h n = 3 (S1/2) or 4 (S1 + S2) mice, analyzed using an b, f, h unpaired Student’s t-test, two-tailed or d a two-way ANOVA with Tukey’s multiple-comparisons test.

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