Fig. 5: aAPC-mediated T cell help is driven by soluble factors and extends to endogenous CD8+ T cells. | Nature Communications

Fig. 5: aAPC-mediated T cell help is driven by soluble factors and extends to endogenous CD8+ T cells.

From: Nanoparticle-based modulation of CD4+ T cell effector and helper functions enhances adoptive immunotherapy

Fig. 5: aAPC-mediated T cell help is driven by soluble factors and extends to endogenous CD8+ T cells.The alternative text for this image may have been generated using AI.

a Epifluorescent imaging and b co-localization analysis of OT-I cells (green) with naïve or Th1 OT-II cells (red) and MHC I/II aAPCs 24 h post-co-incubation. Scale bar: 100 μm. c Transmigration of OT-I cells towards naïve or Th1 OT-II cells relative to the basal medium. d Day 7 intracellular cytokine production of OT-I cells activated alone, separated (sep.) from, or mixed (mix.) with Th1 OT-II cells in a transwell plate. e Cytokine array heatmap depicting secreted proteins from unstimulated or re-stimulated Th1 OT-II cells. f CD127 expression of OT-I cells cocultured with Th1 OT-II cells with blocking antibodies targeting IL-10 and TNF-α. g CD127 expression of OT-I cells stimulated in IL-10 or TNF-α supplemented media. h–j KbSIY, KbOVA, KbTrp2, and Dbgp100 specific CD8+ T cells were enriched from B6 mice and then expanded either alone or in coculture with Th1 OT-II cells. h Representative flow plots, i memory phenotype and j overall cytokine production of antigen-specific CD8+ T cells on day 7. Data in b–d, f, g, i, j represent mean ± standard error of the mean (s.e.m.) from three or more independent experiments. b n = 8, c n = 4 mice, d n = 3 (OT-I + Th1 OT-II sep.) or 5 (OT-I stim, OT-I + Th1 OT-II mix.) mice, e n = 4 mice, f n = 4 (OT-I + Th1 OT-II, OT-I + Th1 OT-II + αTNFα) or 5 (OT-I + Th1 OT-II + αIL-10) mice, g n = 5 (OT-I + TNFα) or 7 (OT-I stim, OT-I + IL-10) mice, and i, j n = 3 mice analyzed using an b unpaired Student’s t-test, two-tailed, c, f, g one-way, or d, e, i, j two-way ANOVA with Tukey’s multiple-comparisons test, mice.

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