Fig. 2: Zfp335cKO DN4 thymocytes undergo increased rates of apoptosis.
From: Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-β selection thymocytes
a, b Assessment of developmental progression throughout OP9-DL1 culture seeded with WT (black) or Zfp335cKO (red) DN3a thymocytes. Proliferation assessment (c, d by Cell Trace Violet (CTV) dilution and apoptosis analysis (e, f) based on Annexin V binding at day 3, 5, or 7 of culture. g Representative comparison of CTV dilution between Annexin V+ and viable (DAPI− Annexin V−) cells on day 5 of culture. h Representative CD4 vs CD8 expression among Annexin V+ cells on day 5 of culture. n = 6 WT or n = 5 Zfp335cKO male and female mice from three independent experiments. P-values determined using two-way repeated-measures ANOVA with post hoc Sidak’s test. Plots show mean ± SEM. Source data are provided as a Source Data file.
