Fig. 2: Urotensin II-saporin treatment affects the breathing pattern and induces hypoxemia during sleep.

Stylized breathing traces based on the average measures of the polysomnography features recorded by whole body plethysmography and coincident EEG recordings in mice injected with control Blank-SAP (A, C) or UII-SAP (B, D) during wake (A, B) or sleep (C, D). Statistics refer to sleep-wake paired two-way ANOVA and Levene tests of variance of both male and female mice. E Average polysomnography measures for individual male mice in UII-SAP or control Blank-SAP groups (sleep-wake paired two-way ANOVA; mean ± s.d) f frequency, PIF peak inspiration flow, PEF the peak exhalation flow, EIP time for inspiration, EEP time for exhalation, RT: F Average blood oxygen saturation level of unrestrained UII-SAP- and Blank-SAP-injected male mice measured during their sleep period (P < 0.0001, Student’s unpaired two-tailed t-test; mean ± s.e.m). G Representative traces of blood oxygen saturation levels of male mice injected with either UII-SAP or control Blank-SAP. *P < 0.05, **P < 0.01, ***P < 0.001 ****P < 0.0001 n.s: non-significant. Each data point represents an individual animal.