Fig. 8: cBF neuronal loss following cMPT lesion is induced by intermittent hypoxia.
A The number of cBF neurons in C67Bl6 mice following 4 weeks of daily sleep-time exposure to hypoxic conditions (P = 0.3780 unpaired two-tailed t-test).). B The percentage of cBF neurons in which HIF1α immunostaining was present in the nucleus in Blank-SAP (gray bar) or UII-SAP mice treated with daily 15 mg/kg 2ME2 (blue bar) or vehicle (red bar) for 3 weeks. (Blank-SAP vs. UII-SAP: ***P = 0.0001, Blank-SAP vs. 2ME2 treated: P = 0.1766, UII-SAP vs. 2ME2 treated: **P = 0.0011). C Representative confocal images of basal forebrain sections from 4 animals immunostained for ChAT (red), HIF1α (green) and nuclei (DAPI; blue). The number of cLDT (D) and cBF (E) neurons in mice following injection of either Blank-SAP (gray bar) or UII-SAP and treated with daily 15 mg/kg 2ME2 (blue bar) or vehicle (red bar) for 3 weeks (cBF results: Blank-SAP vs. UII-SAP: P = 0.0001, Blank-SAP vs. 2ME2 treated: P = 0.0020, UII-SAP vs. 2ME2 treated: P = 0.5401). 2ME2 treatment protects cBF neurons from the effects of lesioning. F Performance of 2ME2-treated (blue bar) and untreated UII-SAP-lesioned (red bar) mice compared to Blank-SAP-lesioned mice (gray bar) in the test phase of the passive place avoidance task. (Blank-SAP vs. UII-SAP: P = 0.0152, Blank-SAP vs. 2ME2 treated: P = 0.4014, UII-SAP vs. 2ME2 treated: *P = 0.0112). G The number of cBF neurons in UII-SAP-injected ChAT-cre HIF-1αfl/wt mice was not significantly different from that in Blank-SAP-injected ChAT-cre HIF-1αfl/wt mice (P = 0.084 unpaired two tailed t-test). * P < 0.05, **P < 0.01, ***P < 0.001 ****P < 0.0001 n.s., non-significant. one-way ANOVA Tukey’s multiple comparison test for panels (B–F). Results are presented as mean ± s.e.m. Each data point represents an individual animal. Source data are provided in the Source Data file.
