Fig. 2: LCR sequences are critical for CPSF6 interaction with HIV-1 cores in infected cells.
From: Prion-like low complexity regions enable avid virus-host interactions during HIV-1 infection
a PLAs showing association of HA-tagged ectopically expressed WT and chimeric CPSF6 proteins with incoming HIV-1 particles in HEK293T CKO cells. The cells were fixed at 6 hpi and PLAs were performed using anti-HA (ab236632, Abcam) and anti-HIV-1 CA specific antibodies (ARP-4121, NIH AIDS Reagent Program). The representative images show PLA puncta (red) and nuclei stained with DAPI (blue). Scale bar is 2 μm. b Quantitative results showing numbers of PLA puncta per cell by analyzing twenty-five cells for each sample. The averaged data (+/− SD) from three independent experiments are shown. Statistical significance of comparison of WT versus the chimeric CPSF6 proteins was determined by Student’s two-sample, two-tailed t-test. CPSF6/AD: p = 3.1E-12; CPSF6/NE: p = 2.4e-12; CPSF6/FU: p = 0.54; CPSF6/CD: p = 0.26; CKO: p = 8.8E-13. P > 0.05 was considered not significant (ns) and p < 0.0001 was considered highly significant (***). Source data are provided as a Source Data file. AD ADD2, NE NEURM, FU FUS, CD CDK19, WT wild-type CPSF6, CKO CPSF6 knock-out, LCR low complexity region.
