Fig. 5: Myeloid deletion of ARIH1 rescues Trex1−/− mice from autoimmune lethality.
a qRT-PCR analysis of Ifnb, Isg15, Isg56, and Ccl5 mRNA in wild type (n = 6), Trex1−/− (n = 6) and Lyz2-Cre;Arih1fl/flTrex1−/− (n = 6) BMDCs (left graphs) or BMDMs (right graphs). b Representative image (left image) and body weight (right graph) of Trex1−/− (n = 11) and Lyz2-Cre;Arih1fl/flTrex1−/− mice (n = 9) (5-week old). c Survival (Kaplan–Meier curve) of Trex1−/− (n = 72) and Lyz2-Cre;Arih1fl/flTrex1−/− (n = 35) mice. d Representative image (left image) of spleen and percentage of spleen/body weight (right graph) of Trex1−/− (n = 9) and Lyz2-Cre;Arih1fl/flTrex1−/− (n = 4) mice (5-week old). e Flow cytometry analysis of splenocytes from Trex1−/− (n = 6) and Lyz2-Cre;Arih1fl/flTrex1−/− mice (n = 6) (5-week old) stained with fluorescence-conjugated antibodies against the indicated surface molecules. f qRT-PCR analysis of Isg15, Isg56, and Ccl5 mRNA in kidney (left graphs) or liver (right graphs) from Trex1−/− (n = 14) and Lyz2-Cre;Arih1fl/flTrex1−/− (n = 6) mice (5-week old). g Representative images and quantification of immune cell infiltration of HE-stained liver, heart and lung sections from Trex1−/− (n = 6) and Lyz2-Cre;Arih1fl/flTrex1−/− (n = 6) mice (5-week old). Arrows indicate infiltrated immune cells. h ELISA analysis of TNF, CXCL1, CCL5, and total IgG in the sera of Trex1−/− (n = 14) and Lyz2-Cre;Arih1fl/flTrex1−/− mice (n = 6) (5-week old). Data are representative of two independent experiments (a, d–h). Graphs show mean ± S.D. (n = 6 for (a, e, g), or n = 9 for (b), or n = 9 and 4 for (d), or n = 14 and 6 for (f, h), biologically independent experiments). Statistical significance was determined using two-tailed Student’s t tests in (b, d–h), or one-way ANOVA in a. Source data are provided as a Source Data file.
