Fig. 3: Increased sensitivity of neutrophils to AZD2811 is accompanied by impaired recovery after drug withdrawal. | Nature Communications

Fig. 3: Increased sensitivity of neutrophils to AZD2811 is accompanied by impaired recovery after drug withdrawal.

From: Differential ABC transporter expression during hematopoiesis contributes to neutrophil-biased toxicity of Aurora kinase inhibitors

Fig. 3

a Human CD34+ progenitor cells were cultured for 10 days and then treated for 48 h with AZD2811 at various concentrations or DMSO vehicle. Cells were harvested and analyzed by flow cytometry to measure cell numbers and the percentage of pH3S10bright cells. Mean and SD are shown; n = 6 for each concentration except n = 3 at highest concentration (5 µM), from 2 independent experiments. ***p = 0.0001 (erythroid) and .0004 (CD34+), ****p < 0.0001, 2-way ANOVA of erythroid and CD34+ cells compared against neutrophils at AZD2811 various concentrations. b Human CD34+ progenitor cells were cultured for 12 days and then treated for 2 h with 1 µM AZD2811 or left untreated. AZD2811 was then washed out and cells were analyzed at the indicated timepoints after washout by intracellular flow cytometry for pH3S10. Representative flow plots are shown; n = 4-6 except 21 h timepoint has 2 data points, from 3 independent experiments. Source data are provided in the Source Data file.

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