Fig. 5: Differences in hematopoietic cell sensitivity to AZD2811 are dependent on ABC transporter function. | Nature Communications

Fig. 5: Differences in hematopoietic cell sensitivity to AZD2811 are dependent on ABC transporter function.

From: Differential ABC transporter expression during hematopoiesis contributes to neutrophil-biased toxicity of Aurora kinase inhibitors

Fig. 5

Human CD34+ progenitor cells were cultured for 10 days and then treated for 48 h with 42 nM AZD2811 or vehicle control. ABC transporter inhibitors (ABCi) targeting B1 (0.5 µM zosuquidar), C1 (50 µM MK-571), and G2 (0.5 µM Ko143) were also added at the time of drug treatment as indicated. a Cells were harvested and pH3S10 was measured by flow cytometry. Representative flow plots are shown along with bar graphs quantitating the percentage of pH3S10bright cells. Mean and SD are shown; n = 6, from 2 independent experiments. ***p = 0.0005, ****p < 0.0001, 1-way ANOVA with post-hoc analysis via Dunnett’s test to compare the AZD-only group against untreated or AZD + ABCi-treated groups. b Cell numbers were measured by flow cytometry. Mean and SD are shown; n = 6, from 2 independent experiments. *p = 0.0137 (neutrophils), *p = 0.0210 (erythroid), ****p < 0.0001, ns (not significant), 1-way ANOVA with post-hoc analysis via Dunnett’s test to compare the AZD-only group against untreated or AZD + ABCi-treated groups. Source data are provided in the Source Data file.

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