Fig. 3: The ribosome is required for pyridazinone formation under in vitro translation conditions.
From: Ribosome-mediated biosynthesis of pyridazinone oligomers in vitro
A The in vitro polymerization reaction was conducted using the same conditions that produced an N-terminal pyridazinone bond in an oligomer, but without the presence of ribosomes. In the deconvoluted mass spectra, the compounds having a molar mass of 179.0701 and 181.1017 were observed as a single peak at 4.4 and 3.6 min, which corresponds to 4-oxo-4-phenylbutanoic acid (orange in B) aminophenylalanine (green in C) hydrolyzed from tRNAfMet(CUA) and tRNAPro1E2(GGU), respectively. D No species corresponding to the theoretical mass of OPDP (322.1317) were observed in the reaction mixture. The extracted ion chromatograms were obtained based on theoretical average masses (Supplementary Information). Spectra are representative of n = 3 independent experiments.
