Fig. 6: Construction of whole blood DNA banking with paper-based cards or 3D-printed artifacts.
From: Long-term whole blood DNA preservation by cost-efficient cryosilicification
a Schematic representation of the preparation of paper-based whole blood genomic DNA banking and its application for forensic identification. b STR analysis of DNA extracted from fresh blood samples and paper-based whole blood DNA bank after aging for 28 days at 70 °C and 60% RH. c Schematic representation of the fabrication of whole blood genomic DNA banking with 3D-printed artifacts (middle, scale bars = 2 cm) and on-demanded DNA extraction. d Microscopy images of cryosilicified leukocytes and erythrocytes in 3D-printed whole blood DNA banking scaffold; Cell nucleus was stained by Hoechst 33342 (blue), and cell membrane was stained by DiO (green), Scale bars = 200 μm. e 3D-printed mouse model. f 3D-printed mouse figurine with embedded cryosilicified mouse blood samples. g Gel electrophoresis of DNA extracted from fresh blood, cryosilicified blood, and 3D-printed DNA banking artifacts before and after its aging for 7 days at 70 °C and 60% RH. A representative image of three biological replicates is shown.
