Fig. 2: Biophysical characterization of berberine and coptisine binding to the KRAS-G4. | Nature Communications

Fig. 2: Biophysical characterization of berberine and coptisine binding to the KRAS-G4.

From: Structural insight into the bulge-containing KRAS oncogene promoter G-quadruplex bound to berberine and coptisine

Fig. 2

a CD thermal melting curves and CD spectra of Pu24m1 DNA with berberine and coptisine, respectively. Conditions: 20 μM DNA, pH 7, 15 mM K+ solution. b Fluorescence intensity change of berberine and coptisine upon titration with Pu24m1 DNA, respectively. Conditions: 0.2 μM compound, pH 7, 50 mM K+ solution. c The determined ΔTm and Kd values of berberine and coptisine to the Pu24m1 DNA. The melting temperature (Tm) was obtained at the intersection between the median of the fitted baselines and the melting curve. The dissociation constant (Kd) was calculated by data fitting with a 2:1 binding equation. The experiments were run in duplicate.

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