Fig. 7: Functional characterization of KRAS-G4. | Nature Communications

Fig. 7: Functional characterization of KRAS-G4.

From: Structural insight into the bulge-containing KRAS oncogene promoter G-quadruplex bound to berberine and coptisine

Fig. 7: Functional characterization of KRAS-G4.The alternative text for this image may have been generated using AI.

a Taq DNA polymerase stop assay using a DNA template containing a wt human KRAS-G4 forming sequence shows that berberine and coptisine can stabilize the KRAS-G4 for replication inhibition. b qRT-PCR results show that berberine (24 µM) and coptisine (10 µM) lower KRAS mRNA levels in both H460 and A549 cancer cells for 24 h. DMSO (<0.1 %) was used as the negative control (no inhibition, 100%). The relative KRAS mRNA levels were normalized with GAPDH. The experiments were run in triplicate. Data are presented as mean values ± SD. P values (**P < 0.01, ***P < 0.001, ****P < 0.0001) were determined by one-way ANOVA with post hoc Dunnett, relative to DMSO control.

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