Fig. 6: Dream-/- mice showed attenuated skin fibrosis. | Nature Communications

Fig. 6: Dream-/- mice showed attenuated skin fibrosis.

From: Fibroblast A20 governs fibrosis susceptibility and its repression by DREAM promotes fibrosis in multiple organs

Fig. 6: Dream-/- mice showed attenuated skin fibrosis.The alt text for this image may have been generated using AI.

Eight-week-old C57/BL6 wild-type mice and DREAM-/- mice were treated in parallel with daily s.c. bleomycin (10 mg/kg/d) or PBS for 2 weeks, and lesional skin was harvested on day 22 for analysis. A Top: schematic of the experiment. Left panels: trichrome stain, representative images. Scale bar: 20 μm. Right panel: dermal thickness (mean ± SD of 5 determinations/hpf, PBS-treated, n = 6, Bleo-treated, n = 8). B Collagen content. One-way ANOVA followed by Sidak’s multiple comparison test. C qPCR. Results, normalized with GAPDH, are mean ± SD of triplicate determinations from 3 mice per group. One-way ANOVA followed by Sidak’s multiple comparison test. D Correlation between skin A20 mRNA levels and dermal thickness. Two-tailed Spearman’s correlation. E Double immunolabeling with antibodies to ASMA/F4/80 and procollagen I/A20. Left panels: representative images; scale bars = 50 µm. Right panels: number of immunopositive cells within the dermis determined at four randomly selected locations/hpf. Results are mean ± SD from three mice/group. One-way ANOVA followed by Sidak’s multiple comparison test.

Back to article page