Fig. 9: Hspanc, but not AtPANC, is required for cyst nematode parasitism. | Nature Communications

Fig. 9: Hspanc, but not AtPANC, is required for cyst nematode parasitism.

From: The genome and lifestage-specific transcriptomes of a plant-parasitic nematode and its host reveal susceptibility genes involved in trans-kingdom synthesis of vitamin B5

Fig. 9: Hspanc, but not AtPANC, is required for cyst nematode parasitism.The alternative text for this image may have been generated using AI.

A Number of female cyst nematodes present per plant root system at 14 dpi (Col-0, n = 16; atpanc, n = 24). B Size of syncytia at 14 dpi. Female-associated syncytia were randomly selected and their outlines were measured (Col-0, n = 28; atpanc, n = 28). C Size of females at 14 dpi. Female nematodes were randomly selected and their outlines were measured (Col-0, n = 28; atpanc, n = 28). A–C Experiments were performed three times independently with a similar outcome. Data from one experiment is shown. Source data are provided as a source data file. Data were analysed using Student’s t test (two-sided; α = 0.05). No significant difference (n.s.) was detected. D Change in transcript abundance of HsPANC gene in J2 nematodes soaked in siRNA targeting HsPANC or GFP. Bars represent mean ± SE. Data were analysed using a one-way analysis of variance (ANOVA) followed by Tukey’s HSD post hoc test (α = 0.05) and different letters indicate significantly different means. Experiments were repeated three times independently (n = 3). E Number of females present per root system at 14 dpi (GFP, n = 9; siRNA1, n = 13; siRNA2 = 13). F Size of females at 14 dpi. Female nematodes were randomly selected and their outlines were measured (GFP, n = 27; siRNA1, n = 32; siRNA2 = 33). G Size of syncytia at 14 dpi. Female-associated syncytia were randomly selected and their outlines were measured (GFP, n = 30; siRNA1, n = 36; siRNA2 = 36). E–G Experiments were performed three times independently with a similar outcome. Data from one experiment is shown. Source data are provided as a source data file. Data were analysed using a one-way analysis of variance (ANOVA) followed by Tukey’s HSD post hoc test (α = 0.05) and different letters indicate significantly different means. (A–C and E–G) Centre line is median; box limits are upper and lower quartiles; whiskers are minimum and maximum value; points are all individual values superimposed on graphs. H) In situ hybridisation of the HsPANC gene in J2 of cyst nematode.

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