Fig. 2: A subset of ex vivo differentiated PCs resemble bone marrow ASCs. | Nature Communications

Fig. 2: A subset of ex vivo differentiated PCs resemble bone marrow ASCs.

From: Ex vivo engineered human plasma cells exhibit robust protein secretion and long-term engraftment in vivo

Fig. 2

a, b Using the indicated protein markers: active B (CD19hiCD38-), CD38+, CD38++, and CD138hi flow cytometry showing expression of MitoTracker Green FM (mitochondrial volume) and MitoTracker Red CMXRos (mitochondrial stress) from different B cell subsets. The data (n = 5 subjects) are normalized by minimal and maximal intensity per replicate and presented as mean and 95% confidence intervals. To assess significance, we used a paired one-way ANOVA with Tukey’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001). P values for CD38+ and CD138hi with MitoTracker Green FM is 0.0123, for CD38+ and CD38++, CD38+ and CD138hi with MitoTracker Red CMXRos are 0.0044 and 0.0041, respectively. Source data are provided as a Source Data file. c Transmission electron microscopy of day 2 and day 13 B cells from three-stage culture. d Quantification of rough endoplasmic reticulum (RER) in ex vivo differentiated cultures. Day 2 (43 images) and day 13 cells (60 images) were scored blindly for having prominent RER or not (n = 6 participants). Data were presented as mean ± SD. Source data are provided as a Source Data file.

Back to article page