Fig. 7: LRP1 plays a crucial role regulating VWF inflammatory signaling.

a Flow cytometry was used to determine the binding of pd-VWF to human primary macrophages in the presence of LRP1 blocking antibody. Flow gating strategy is presented in Supplementary Fig. 14. Representative histogram of VWF bound to macrophage (blue) and pd-VWF bound to macrophage in the presence of anti-LRP1 antibody (black). b Inhibition of human macrophage LRP1 binding with receptor associated protein (RAP) decreased p38 phosphorylation by 50% (*P = 0.013), with no alteration in JNK or NF-κB activation (ns = not significant, P = 0.642 and P = 0.374, respectively). c Densitometry of western blots determined from 3 independent experiments. d Inhibition of LRP1 using a monoclonal anti-LRP1 antibody resulted in similar effects as RAP inhibition with p38 signaling reduced 50% (*P = 0.0394), and no alteration in NK-κB activation (P = 0.953). e Densitometry of western blots determined from three independent experiments (mean ± SD). The significance was determined by Mann Whitney or t-test in which *P < 0.05. Source data for this figure are provided as a Source Data file.