Fig. 1: Proteomics screen to study proteome expression in a knockout library. | Nature Communications

Fig. 1: Proteomics screen to study proteome expression in a knockout library.

From: Proteome effects of genome-wide single gene perturbations

Fig. 1

a Schematics of the experimental design for quantifying protein expression levels in the S. pombe knockout collection by quantitative proteomics using 10plex TMT. Each MS run contained a technical (127 C label) and a biological control (130 N label), while the biological control samples were grown along with the knockout samples. b Normalized protein expression levels of 3308 knockout strains and 461 wild-type replicates. The complete dataset contains quantification of 2921 protein-coding genes, of which 513 proteins were quantified across all samples. Source data are provided as a Source Data file.

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