Fig. 5: Smut Erc1 prevents induction of host defenses.
From: A conserved enzyme of smut fungi facilitates cell-to-cell extension in the plant bundle sheath
a A ROS-burst assay was performed with barley leaf disks following incubation with laminarihexaose, Erc1, Erc1M2x, and a mix of laminarihexaose and Erc1 recombinant proteins. BSA was used as a negative control for background signal. Relative luminescence units (RLU) indicate ROS burst activity of treated barley leaf discs. The RLU are normalized with the buffer control. Each curve shows the mean of at least nine technical leaf discs with three independent biological experiments. Data are presented as mean value ± SD. b Statistical analysis was performed with sum of RLU values of each sample. Data are presented as mean value ± SD. Asterisks above bars indicate significant differences (p < 0.05, two-tailed student’s t test). c RT-qPCR for pathogenesis related (PR) gene expression on SG200 and SG200∆erc1 mutant strains infected maize leaves at 4 days post infection (dpi). The expression levels of maize PR genes, including PR1, PR3, PR4, PR5, and PRm6b were calculated relative to the GAPDH gene of maize. Data are presented as mean value ± SD. Asterisks above bars indicate significant differences (p < 0.05, pairwise Kruskal-Wallis H-test). Calculated p values are shown in the Source Data.
