Fig. 4: Intestinal DAF-16 mediates daf-2(e1370) longevity. | Nature Communications

Fig. 4: Intestinal DAF-16 mediates daf-2(e1370) longevity.

From: Intestine-specific removal of DAF-2 nearly doubles lifespan in Caenorhabditis elegans with little fitness cost

Fig. 4: Intestinal DAF-16 mediates daf-2(e1370) longevity.

a Degrading DAF-16 from the neurons shortened the daf-2(e1370) lifespan by 15.6% (p < 0.0001). b Degrading DAF-16 from the germline had no significant effect on the daf-2(e1370) lifespan (p = 0.347). c Degrading DAF-16 from the hypodermis shortened the daf-2(e1370) lifespan by 15.6% (p < 0.0001). Degrading DAF-16 in the body wall muscle (d), gonadal sheath (e), or XXX cells (f) had no effect on the lifespan of daf-2(e1370) (p > 0.05). g Degrading intestinal DAF-16 shortened the daf-2(e1370) lifespan by 40.1% (p < 0.0001). h Degrading DAF-16 in all tissues shortened the daf-2(e1370) lifespan by 57.8% (p < 0.0001). i Endogenously expressed DAF-16::GFP accumulated in the intestinal nuclei upon degrading DAF-2 from the intestine. A similar pattern of expression was observed in three independent experiments. j Degrading intestinal DAF-16 largely suppressed the longevity induced by degrading DAF-2 from the intestine (red dashed line versus red solid line, p < 0.0001). p values are calculated by log-rank tests. See survival statistics in Supplementary Data 1. Source data are provided as a Source Data file.

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