Fig. 4: Effect of primary sequence of the S/T-P sites on the c-JUN TAD N-terminal phosphorylation kinetics. | Nature Communications

Fig. 4: Effect of primary sequence of the S/T-P sites on the c-JUN TAD N-terminal phosphorylation kinetics.

From: An intrinsic temporal order of c-JUN N-terminal phosphorylation regulates its activity by orchestrating co-factor recruitment

Fig. 4

a Schematic representation of the c-JUN TAD constructs swap and TTSS comparing to wild-type (WT). The initial rate of phosphorylation of residues by JNK1, determined by time-resolved NMR spectroscopy, is indicated according to the displayed colour scale. b Site-specific build-up curves of the phosphorylation of c-JUN swap variant by JNK1. Data are plotted as the mean ± standard error of the mean (SEM) (n = 3 biologically independent samples) and fitted to single exponential build-up curves; error bars are presented as ribbons and pale lines show fits to wild-type c-JUN TAD (Fig. 1c) for comparison. c Site-specific build-up curves of the phosphorylation of c-JUN TTSS variant by JNK1. Data are plotted as the mean of two biologically independent samples and fitted to single exponential build-up curves; pale lines show fits to wild-type c-JUN TAD (Fig. 1c) for comparison. Source data are provided as a Source Data file.

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